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10 minutes maximum! Can you do it in 5? |
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Q1-3: Different varieties of Morning glory plants produce flowers of different colours. Two different varieties are shown in the photograph.
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![]() PiccoloNamek | CC 3.0 |
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| 1. A gardener likes the purple variety of the morning glory plant and takes 6 cuttings from one plant. The cuttings grows into 6 new plants.
This is an example of ..
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2. Which of the following statements is incorrect? The new plants will have ..
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3. Which of the following is not an advantage of cloning plants?
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Q4-6: Dolly was the first successfully cloned mammal. |
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4. Dolly’s genome comes from ...
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5. An enucleated cell is a cell that ...
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6. What does the term transgenic mean?
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Q7-10: Tissue culture or micropropagation of plant tissue can be used to propagate plants. Often these plants may have been genetically engineered to include a beneficial gene. The image shows one of the stages of micropropagation.
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![]() Syed Sajidul Islam | CC 4.0 |
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7. The stages of tissue culture include:
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The correct order of the stages is
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8. The explants are grown on a medium that contains nutrients and growth regulators, as shown in this table:
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Why are magnesium ions included?
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9. Which of the following is needed for a high rate of growth of the explants?
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10. Which of the following is NOT an advantage of tissue culture?
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Question 1:
The correct answer is A. cloning.
Explanation:
Taking cuttings from one plant to produce new plants that are genetically identical to the parent is a form of asexual reproduction, resulting in clones (genetically identical individuals).
A. cloning – Correct. The new plants are clones of the original.
B. selective breeding – Involves choosing parent plants with desired traits and crossing them sexually over generations; not what is happening here.
C. genetic modification – Involves directly altering the DNA using biotechnology; not described.
D. sexual reproduction – Involves gametes (pollen and ovules) and produces genetic variation; cuttings bypass this.
Thus, A is the correct answer.
*These A.I. responses have been individually checked to ensure they match the accepted answer, but explanations may still be incorrect. Responses may give guidance but the A.I. might not be able to answer the question! This is particularly the case for questions based on diagrams, which the A.I. typically cannot interpret.
Grade Gorilla uses Gemini, Deepseek and a range of other A.I. chatbots to generate the saved responses. Some answers have had human intervention for clarity or where the A.I. has not been able to answer the question.
Question 2:
The correct answer is D. mixture of purple and pink flowers.
Explanation:
Since the new plants were produced by cuttings (asexual reproduction / cloning), they are genetically identical to the purple parent plant.
A. Same number of chromosomes as the parent plant – True; clones have the same chromosome number.
B. Same alleles as the parent plant – True; they are genetically identical, so they carry the same alleles.
C. Only purple flowers – True; the parent is purple and has only purple alleles (assuming pure-breeding for that trait), so clones will also be purple.
D. Mixture of purple and pink flowers – False; there will be no mixture because no sexual reproduction or genetic variation occurred. All flowers will be purple like the parent.
Thus, D is the incorrect statement.
*These A.I. responses have been individually checked to ensure they match the accepted answer, but explanations may still be incorrect. Responses may give guidance but the A.I. might not be able to answer the question! This is particularly the case for questions based on diagrams, which the A.I. typically cannot interpret.
Grade Gorilla uses Gemini, Deepseek and a range of other A.I. chatbots to generate the saved responses. Some answers have had human intervention for clarity or where the A.I. has not been able to answer the question.
Question 3:
The correct answer is C. Lots of different varieties of the parent plant are produced.
Explanation:
Cloning produces genetically identical offspring (copies of the parent). This means no new varieties are created — in fact, a lack of genetic variation is a disadvantage, not an advantage, of cloning.
A. It can be done at any time of the year – This can be an advantage (e.g., in tissue culture or taking cuttings under controlled conditions).
B. Large numbers of plants produced – Advantage; cloning can rapidly produce many plants.
C. Lots of different varieties of the parent plant are produced – Not an advantage of cloning; in fact, cloning does not produce different varieties. This statement is false for cloning.
D. All plants are identical – Can be an advantage if uniformity is desired (e.g., in crops for consistent yield or quality).
Thus, C is the correct choice because it incorrectly describes an advantage of cloning.
*These A.I. responses have been individually checked to ensure they match the accepted answer, but explanations may still be incorrect. Responses may give guidance but the A.I. might not be able to answer the question! This is particularly the case for questions based on diagrams, which the A.I. typically cannot interpret.
Grade Gorilla uses Gemini, Deepseek and a range of other A.I. chatbots to generate the saved responses. Some answers have had human intervention for clarity or where the A.I. has not been able to answer the question.
Question 4:
Dolly's genome comes from the female Finn-Dorset sheep (Option B).
Why?
Nuclear Donor: The diagram labels the Finn-Dorset as the "Nuclear Donor." In cloning, the nucleus contains the vast majority of the organism's genetic material (DNA).
Source Cells: The nucleus was taken from the mammary cells of the Finn-Dorset sheep.
Other Roles: The Scottish Blackface provided only the egg cytoplasm (with its nucleus removed), and the surrogate ewe provided the womb for development, but neither contributed to Dolly's nuclear genome.
*These A.I. responses have been individually checked to ensure they match the accepted answer, but explanations may still be incorrect. Responses may give guidance but the A.I. might not be able to answer the question! This is particularly the case for questions based on diagrams, which the A.I. typically cannot interpret.
Grade Gorilla uses Gemini, Deepseek and a range of other A.I. chatbots to generate the saved responses. Some answers have had human intervention for clarity or where the A.I. has not been able to answer the question.
Question 5:
The correct answer is D. has no nucleus.
Explanation:
The prefix "enucleated" comes from *e-* (without) + nucleus → without a nucleus. In biology, an enucleated cell has had its nucleus removed.
A. has a haploid nucleus – Incorrect; haploid means one set of chromosomes, not no nucleus.
B. has a diploid nucleus – Incorrect; diploid means two sets, still has a nucleus.
C. has a normal nucleus – Opposite of enucleated.
D. has no nucleus – Correct.
*These A.I. responses have been individually checked to ensure they match the accepted answer, but explanations may still be incorrect. Responses may give guidance but the A.I. might not be able to answer the question! This is particularly the case for questions based on diagrams, which the A.I. typically cannot interpret.
Grade Gorilla uses Gemini, Deepseek and a range of other A.I. chatbots to generate the saved responses. Some answers have had human intervention for clarity or where the A.I. has not been able to answer the question.
Question 6:
The correct answer is A. Transfer of material from one species to a different species.
Explanation:
Transgenic refers to an organism that has had a gene (or genes) from another species inserted into its genome using genetic engineering techniques. The inserted gene is called a transgene, and the organism is said to be transgenic.
A. Transfer of genetic material from one species to a different species – Correct definition of transgenic.
B. Production of genetically identical organisms by asexual reproduction – This describes cloning.
C. Mating two individuals of different sexes – This is normal sexual reproduction.
D. Changing sex from a male to a female – This is sex reversal, not related to transgenics.
Thus, A is correct.
*These A.I. responses have been individually checked to ensure they match the accepted answer, but explanations may still be incorrect. Responses may give guidance but the A.I. might not be able to answer the question! This is particularly the case for questions based on diagrams, which the A.I. typically cannot interpret.
Grade Gorilla uses Gemini, Deepseek and a range of other A.I. chatbots to generate the saved responses. Some answers have had human intervention for clarity or where the A.I. has not been able to answer the question.
Question 7:
Let’s reason through the correct sequence for tissue culture / micropropagation:
Obtain explant → Take a small piece of plant tissue (explant).
Sterilize explant → Often with bleach to kill microbes (explant sterilised with bleach).
Grow explant under sterile conditions → Placed on nutrient medium to form callus (undifferentiated cell mass).
Callus divides → Callus subcultured to produce many plantlets (division of callus).
Grow in controlled conditions before planting outside → Acclimatization step.
Order from the given steps:
Start with removal/sterilization (3) → then growth under sterile conditions (1) → then division of callus (4) → then grow before planting (2).
That matches 3 → 1 → 4 → 2, which is option D.
✅ Correct answer: D
*These A.I. responses have been individually checked to ensure they match the accepted answer, but explanations may still be incorrect. Responses may give guidance but the A.I. might not be able to answer the question! This is particularly the case for questions based on diagrams, which the A.I. typically cannot interpret.
Grade Gorilla uses Gemini, Deepseek and a range of other A.I. chatbots to generate the saved responses. Some answers have had human intervention for clarity or where the A.I. has not been able to answer the question.
Question 8:
The correct answer is B. Help plantlets make chlorophyll.
Explanation:
Magnesium ions (Mg²⁺) are an essential component of the chlorophyll molecule (the central atom in the chlorophyll ring). Without magnesium, plants cannot produce chlorophyll and therefore cannot photosynthesize effectively.
A. Stop bacteria growing – No; that would be antibiotics or sterilizing agents.
B. Help plantlets make chlorophyll – Yes; magnesium is a key component of chlorophyll.
C. Help plantlets respire – No; respiration mainly involves glucose, oxygen, and enzymes, though magnesium plays minor roles in enzyme activation, it is not primarily for respiration.
D. Help plantlets make proteins – No; protein synthesis primarily requires nitrates (for amino acids) and other elements like sulfur, but magnesium is not directly required for protein synthesis.
Thus, B is correct.
*These A.I. responses have been individually checked to ensure they match the accepted answer, but explanations may still be incorrect. Responses may give guidance but the A.I. might not be able to answer the question! This is particularly the case for questions based on diagrams, which the A.I. typically cannot interpret.
Grade Gorilla uses Gemini, Deepseek and a range of other A.I. chatbots to generate the saved responses. Some answers have had human intervention for clarity or where the A.I. has not been able to answer the question.
Question 9:
The correct answer is B. High light intensity and warm conditions.
Explanation:
For high growth rates of explants (plant tissue) in tissue culture:
Light – Provides energy for photosynthesis (once shoots/chlorophyll develop). High light intensity generally promotes growth, though very early callus stages may require lower light initially, but for overall growth of plantlets, high light + warm is optimal.
Warm conditions – Increases the rate of enzyme-controlled reactions, including cell division and metabolism, up to an optimum (typically ~20–25°C for many plants).
A. Air with 100% oxygen and warm conditions – 100% oxygen is not needed; normal air (~21% oxygen) is sufficient. Too much oxygen can be toxic.
C. Cold temperature and high light intensity – Cold temperatures slow down metabolic processes and growth.
D. Low light intensity and warm conditions – Low light limits photosynthesis, reducing growth rates.
Thus, B is best.
*These A.I. responses have been individually checked to ensure they match the accepted answer, but explanations may still be incorrect. Responses may give guidance but the A.I. might not be able to answer the question! This is particularly the case for questions based on diagrams, which the A.I. typically cannot interpret.
Grade Gorilla uses Gemini, Deepseek and a range of other A.I. chatbots to generate the saved responses. Some answers have had human intervention for clarity or where the A.I. has not been able to answer the question.
Question 10:
The correct answer is D. All plants produced will be susceptible to the same disease.
Explanation:
This statement is not an advantage — it is actually a major disadvantage of tissue culture (and any form of cloning). Since all plants are genetically identical, a disease that affects one plant can wipe out the entire crop because there is no genetic variation to provide resistance.
A. Used to preserve rare plant species – Advantage; tissue culture helps conserve endangered plants by producing many individuals.
B. Produce large numbers of genetically identical plants with desirable characteristics – Advantage; uniformity is useful in agriculture and horticulture.
C. Reproduction of plants is possible without having to wait for the onset of seed production – Advantage; allows rapid propagation anytime.
D. All plants produced will be susceptible to the same disease – Not an advantage; this is a risk of genetic uniformity.
Thus, D is correct.
*These A.I. responses have been individually checked to ensure they match the accepted answer, but explanations may still be incorrect. Responses may give guidance but the A.I. might not be able to answer the question! This is particularly the case for questions based on diagrams, which the A.I. typically cannot interpret.
Grade Gorilla uses Gemini, Deepseek and a range of other A.I. chatbots to generate the saved responses. Some answers have had human intervention for clarity or where the A.I. has not been able to answer the question.